Reconstitution of a core chromatin remodeling complex from SWI/SNF subunits.
Journal:
Mol. Cell 1999 FebAuthors:
Phelan ML, Sif S, Narlikar GJ, Kingston RE
Abstract
Protein complexes of the SWI/SNF family remodel nucleosome structure in an ATP-dependent manner. Each complex contains between 8 and 15 subunits, several of which are highly conserved between yeast, Drosophila, and humans. We have reconstituted an ATP-dependent chromatin remodeling complex using a subset of conserved subunits. Unexpectedly, both BRG1 and hBRM, the ATPase subunits of human SWI/SNF complexes, are capable of remodeling mono-nucleosomes and nucleosomal arrays as purified proteins. T
...[more]he addition of INI1, BAF155, and BAF170 to BRG1 increases remodeling activity to a level comparable to that of the whole hSWI/SNF complex. These data define the functional core of the hSWI/SNF complex.[less]
Mesh Headings:
Actins, Adenosine Triphosphate, Cell-Free System, Chromatin, Chromosomal Proteins, Non-Histone, DNA Helicases, DNA-Binding Proteins, Drosophila Proteins, Evolution, Molecular, Humans, Macromolecular Substances, Nuclear Proteins, Nucleosomes, RNA-Binding Proteins, Recombinant Fusion Proteins, Ribonucleoprotein, U1 Small Nuclear, Transcription Factors