Differential roles of TLR2 and TLR4 in recognition of gram-negative and gram-positive bacterial cell wall components.
Journal:
Immunity 1999 OctAuthors:
Takeuchi O, Hoshino K, Kawai T, Sanjo H, Takada H, Ogawa T, Takeda K, Akira S
Abstract
Toll-like receptor (TLR) 2 and TLR4 are implicated in the recognition of various bacterial cell wall components, such as lipopolysaccharide (LPS). To investigate in vivo roles of TLR2, we generated TLR2-deficient mice. In contrast to LPS unresponsiveness in TLR4-deficient mice, TLR2-deficient mice responded to LPS to the same extent as wild-type mice. TLR2-deficient macrophages were hyporesponsive to several Gram-positive bacterial cell walls as well as Staphylococcus aureus peptidoglycan. TLR4-
...[more]deficient macrophages lacked the response to Gram-positive lipoteichoic acids. These results demonstrate that TLR2 and TLR4 recognize different bacterial cell wall components in vivo and TLR2 plays a major role in Gram-positive bacterial recognition.[less]
Mesh Headings:
Animals, Antigens, Bacterial, Cell Wall, Corynebacterium diphtheriae, Drosophila Proteins, Escherichia coli, Female, Gram-Negative Bacteria, Gram-Positive Bacteria, Interleukin-1 Receptor-Associated Kinases, Lipid A, Lipopolysaccharides, Macrophages, Peritoneal, Male, Membrane Glycoproteins, Mice, Mice, Inbred C57BL, Mice, Knockout, NF-kappa B, Nocardia, Peptidoglycan, Protein Isoforms, Protein Kinases, Receptors, Cell Surface, Salmonella, Signal Transduction, Species Specificity, Staphylococcus aureus, Teichoic Acids, Toll-Like Receptor 2, Toll-Like Receptor 4, Toll-Like Receptors