• Register
X
Forgot Password

If you have forgotten your password you can enter your email here and get a temporary password sent to your email.

X

Leaving Community

Are you sure you want to leave this community? Leaving the community will revoke any permissions you have been granted in this community.

No
Yes

Differential roles of TLR2 and TLR4 in recognition of gram-negative and gram-positive bacterial cell wall components.

Toll-like receptor (TLR) 2 and TLR4 are implicated in the recognition of various bacterial cell wall components, such as lipopolysaccharide (LPS). To investigate in vivo roles of TLR2, we generated TLR2-deficient mice. In contrast to LPS unresponsiveness in TLR4-deficient mice, TLR2-deficient mice responded to LPS to the same extent as wild-type mice. TLR2-deficient macrophages were hyporesponsive to several Gram-positive bacterial cell walls as well as Staphylococcus aureus peptidoglycan. TLR4-deficient macrophages lacked the response to Gram-positive lipoteichoic acids. These results demonstrate that TLR2 and TLR4 recognize different bacterial cell wall components in vivo and TLR2 plays a major role in Gram-positive bacterial recognition.

Pubmed ID: 10549626

Authors

  • Takeuchi O
  • Hoshino K
  • Kawai T
  • Sanjo H
  • Takada H
  • Ogawa T
  • Takeda K
  • Akira S

Journal

Immunity

Publication Data

October 23, 1999

Associated Grants

None

Mesh Terms

  • Animals
  • Antigens, Bacterial
  • Cell Wall
  • Corynebacterium diphtheriae
  • Drosophila Proteins
  • Escherichia coli
  • Female
  • Gram-Negative Bacteria
  • Gram-Positive Bacteria
  • Interleukin-1 Receptor-Associated Kinases
  • Lipid A
  • Lipopolysaccharides
  • Macrophages, Peritoneal
  • Male
  • Membrane Glycoproteins
  • Mice
  • Mice, Inbred C57BL
  • Mice, Knockout
  • NF-kappa B
  • Nocardia
  • Peptidoglycan
  • Protein Isoforms
  • Protein Kinases
  • Receptors, Cell Surface
  • Salmonella
  • Signal Transduction
  • Species Specificity
  • Staphylococcus aureus
  • Teichoic Acids
  • Toll-Like Receptor 2
  • Toll-Like Receptor 4
  • Toll-Like Receptors