Roles of PLC-beta2 and -beta3 and PI3Kgamma in chemoattractant-mediated signal transduction.
Journal:
Science 2000 FebAuthors:
Li Z, Jiang H, Xie W, Zhang Z, Smrcka AV, Wu D
Abstract
The roles of phosphoinositide 3-kinase (PI3K) and phospholipase C (PLC) in chemoattractant-elicited responses were studied in mice lacking these key enzymes. PI3Kgamma was required for chemoattractant-induced production of phosphatidylinositol 3,4,5-trisphosphate [PtdIns (3,4,5)P3] and has an important role in chemoattractant-induced superoxide production and chemotaxis in mouse neutrophils and in production of T cell-independent antigen-specific antibodies composed of the immunoglobulin lambda
...[more]light chain (TI-IglambdaL). The study of the mice lacking PLC-beta2 and -beta3 revealed that the PLC pathways have an important role in chemoattractant-mediated production of superoxide and regulation of protein kinases, but not chemotaxis. The PLC pathways also appear to inhibit the chemotactic activity induced by certain chemoattractants and to suppress TI-IglambdaL production.[less]
Mesh Headings:
Animals, B-Lymphocytes, Chemokine CCL4, Chemotactic Factors, Chemotaxis, Leukocyte, Immunoglobulin lambda-Chains, Isoenzymes, Macrophage Inflammatory Proteins, Mice, N-Formylmethionine Leucyl-Phenylalanine, Neutrophil Infiltration, Neutrophils, Peritonitis, Phosphatidylinositol 3-Kinases, Phosphatidylinositol Phosphates, Phospholipase C beta, Phosphorylation, Signal Transduction, Skin Ulcer, Superoxides, Type C Phospholipases