• Register
X
Forgot Password

If you have forgotten your password you can enter your email here and get a temporary password sent to your email.

X

Leaving Community

Are you sure you want to leave this community? Leaving the community will revoke any permissions you have been granted in this community.

No
Yes

Frizzled regulates localization of cell-fate determinants and mitotic spindle rotation during asymmetric cell division.

Cell-fate diversity is generated in part by the unequal segregation of cell-fate determinants during asymmetric cell divisions. In the Drosophila pupa, the pI sense organ precursor cell is polarized along the anterior-posterior axis of the fly and divides asymmetrically to generate a posterior pIIa cell and an anterior pIIb cell. The anterior pIIb cell specifically inherits the determinant Numb and the adaptor protein Partner of Numb (Pon). By labelling both the Pon crescent and the microtubules in living pupae, we show that determinants localize at the anterior cortex before mitotic-spindle formation, and that the spindle forms with random orientation and rotates to line up with the Pon crescent. By imaging living frizzled (fz) mutant pupae we show that Fz regulates the orientation of the polarity axis of pI, the initiation of spindle rotation and the unequal partitioning of determinants. We conclude that Fz participates in establishing the polarity of pI.

Pubmed ID: 11146626

Authors

  • Bella├»che Y
  • Gho M
  • Kaltschmidt JA
  • Brand AH
  • Schweisguth F

Journal

Nature cell biology

Publication Data

January 26, 2001

Associated Grants

None

Mesh Terms

  • Animals
  • Body Patterning
  • Carrier Proteins
  • Cell Division
  • Cell Lineage
  • Cell Polarity
  • Centrosome
  • Drosophila
  • Drosophila Proteins
  • Frizzled Receptors
  • Green Fluorescent Proteins
  • Indicators and Reagents
  • Juvenile Hormones
  • Luminescent Proteins
  • Membrane Proteins
  • Microtubules
  • Neurons, Afferent
  • Prophase
  • Pupa
  • Receptors, G-Protein-Coupled
  • Rotation
  • Sense Organs
  • Signal Transduction
  • Spindle Apparatus