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The zinc-finger proto-oncogene Gfi-1b is essential for development of the erythroid and megakaryocytic lineages.

Gfi-1 and Gfi-1b are novel proto-oncogenes identified by retroviral insertional mutagenesis. By gene targeting, we establish that Gfi-1b is required for the development of two related blood lineages, erythroid and megakaryocytic, in mice. Gfi-1b(-/-) embryonic stem cells fail to contribute to red cells of adult chimeras. Gfi-1b(-/-) embryos exhibit delayed maturation of primitive erythrocytes and subsequently die with failure to produce definitive enucleated erythrocytes. The fetal liver of mutant mice contains erythroid and megakaryocytic precursors arrested in their development. Myelopoiesis is normal. Therefore, Gfi-1b is an essential transcriptional regulator of erythroid and megakaryocyte development.

Pubmed ID: 11825872

Authors

  • Saleque S
  • Cameron S
  • Orkin SH

Journal

Genes & development

Publication Data

February 1, 2002

Associated Grants

None

Mesh Terms

  • Animals
  • Cell Lineage
  • Embryo, Mammalian
  • Erythrocytes
  • Erythropoiesis
  • Flow Cytometry
  • Gene Expression
  • Hematopoiesis
  • Megakaryocytes
  • Mice
  • Mice, Inbred C57BL
  • Mice, Knockout
  • Proto-Oncogene Proteins
  • Repressor Proteins
  • Reverse Transcriptase Polymerase Chain Reaction
  • Zinc Fingers