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A complex with chromatin modifiers that occupies E2F- and Myc-responsive genes in G0 cells.

E2F-6 contributes to gene silencing in a manner independent of retinoblastoma protein family members. To better elucidate the molecular mechanism of repression by E2F-6, we have purified the factor from cultured cells. E2F-6 is found in a multimeric protein complex that contains Mga and Max, and thus the complex can bind not only to the E2F-binding site but also to Myc- and Brachyury-binding sites. Moreover, the complex contains chromatin modifiers such as a novel histone methyltransferase that modifies lysine 9 of histone H3, HP1gamma, and Polycomb group (PcG) proteins. The E2F-6 complex preferentially occupies target promoters in G0 cells rather than in G1 cells. These data suggest that these chromatin modifiers contribute to silencing of E2F- and Myc-responsive genes in quiescent cells.

Pubmed ID: 12004135

Authors

  • Ogawa H
  • Ishiguro K
  • Gaubatz S
  • Livingston DM
  • Nakatani Y

Journal

Science (New York, N.Y.)

Publication Data

May 10, 2002

Associated Grants

None

Mesh Terms

  • Amino Acid Sequence
  • Binding Sites
  • Cell Cycle Proteins
  • Chromatin
  • Chromosomal Proteins, Non-Histone
  • DNA-Binding Proteins
  • Dimerization
  • E2F Transcription Factors
  • E2F6 Transcription Factor
  • G0 Phase
  • G1 Phase
  • Gene Silencing
  • HeLa Cells
  • Histone-Lysine N-Methyltransferase
  • Histones
  • Humans
  • Mass Spectrometry
  • Methylation
  • Methyltransferases
  • Molecular Sequence Data
  • Phosphoproteins
  • Promoter Regions, Genetic
  • Protein Footprinting
  • Protein Methyltransferases
  • Proteins
  • Proto-Oncogene Proteins c-myc
  • Recombinant Proteins
  • Retinoblastoma-Like Protein p130
  • Transcription Factors
  • Transcription, Genetic
  • Two-Hybrid System Techniques