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Pex8p: an intraperoxisomal organizer of the peroxisomal import machinery.

Peroxisomes transport folded and oligomeric proteins across their membrane. Two cytosolic import receptors, Pex5p and Pex7p, along with approximately 12 membrane-bound peroxins participate in this process. While interactions among individual peroxins have been described, their organization into functional units has remained elusive. We have purified and defined two core complexes of the peroxisomal import machinery: the docking complex comprising Pex14p and Pex17p, with the loosely associated Pex13p, and the RING finger complex containing Pex2p, Pex10p, and Pex12p. Association of both complexes into a larger import complex requires Pex8p, an intraperoxisomal protein. We conclude that Pex8p organizes the formation of the larger import complex from the trans side of the peroxisomal membrane and thus might enable functional communication between both sides of the membrane.

Pubmed ID: 12667447

Authors

  • Agne B
  • Meindl NM
  • Niederhoff K
  • Einw├Ąchter H
  • Rehling P
  • Sickmann A
  • Meyer HE
  • Girzalsky W
  • Kunau WH

Journal

Molecular cell

Publication Data

March 1, 2003

Associated Grants

None

Mesh Terms

  • Carrier Proteins
  • Cell Membrane
  • Cytosol
  • Electrophoresis, Polyacrylamide Gel
  • Fungal Proteins
  • Membrane Proteins
  • Membrane Transport Proteins
  • Models, Biological
  • Peroxisomes
  • Plasmids
  • Protein Binding
  • Receptors, Cytoplasmic and Nuclear
  • Recombinant Fusion Proteins
  • Repressor Proteins
  • Saccharomyces cerevisiae
  • Saccharomyces cerevisiae Proteins
  • Ubiquitin-Protein Ligases