Design of a novel globular protein fold with atomic-level accuracy.
Journal:
Science 2003 NovAuthors:
Kuhlman B, Dantas G, Ireton GC, Varani G, Stoddard BL, Baker D
Abstract
A major challenge of computational protein design is the creation of novel proteins with arbitrarily chosen three-dimensional structures. Here, we used a general computational strategy that iterates between sequence design and structure prediction to design a 93-residue alpha/beta protein called Top7 with a novel sequence and topology. Top7 was found experimentally to be folded and extremely stable, and the x-ray crystal structure of Top7 is similar (root mean square deviation equals 1.2 angstro
...[more]ms) to the design model. The ability to design a new protein fold makes possible the exploration of the large regions of the protein universe not yet observed in nature.[less]
Mesh Headings:
Algorithms, Amino Acid Sequence, Circular Dichroism, Computational Biology, Computer Graphics, Computer Simulation, Crystallization, Crystallography, X-Ray, Databases, Protein, Models, Molecular, Molecular Sequence Data, Monte Carlo Method, Nuclear Magnetic Resonance, Biomolecular, Protein Conformation, Protein Denaturation, Protein Engineering, Protein Folding, Protein Structure, Secondary, Proteins, Software, Solubility, Temperature, Thermodynamics