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RNAi-mediated targeting of heterochromatin by the RITS complex.

RNA interference (RNAi) is a widespread silencing mechanism that acts at both the posttranscriptional and transcriptional levels. Here, we describe the purification of an RNAi effector complex termed RITS (RNA-induced initiation of transcriptional gene silencing) that is required for heterochromatin assembly in fission yeast. The RITS complex contains Ago1 (the fission yeast Argonaute homolog), Chp1 (a heterochromatin-associated chromodomain protein), and Tas3 (a novel protein). In addition, the complex contains small RNAs that require the Dicer ribonuclease for their production. These small RNAs are homologous to centromeric repeats and are required for the localization of RITS to heterochromatic domains. The results suggest a mechanism for the role of the RNAi machinery and small RNAs in targeting of heterochromatin complexes and epigenetic gene silencing at specific chromosomal loci.

Pubmed ID: 14704433

Authors

  • Verdel A
  • Jia S
  • Gerber S
  • Sugiyama T
  • Gygi S
  • Grewal SI
  • Moazed D

Journal

Science (New York, N.Y.)

Publication Data

January 30, 2004

Associated Grants

  • Agency: NIGMS NIH HHS, Id: R01 GM072805
  • Agency: NIGMS NIH HHS, Id: R01 GM072805-01
  • Agency: Howard Hughes Medical Institute, Id:

Mesh Terms

  • Amino Acid Sequence
  • Argonaute Proteins
  • Cell Cycle Proteins
  • Centromere
  • Chromosomes, Fungal
  • Endoribonucleases
  • Genes, Reporter
  • Heterochromatin
  • Mass Spectrometry
  • Models, Genetic
  • Molecular Sequence Data
  • Mutation
  • Precipitin Tests
  • Protein Binding
  • RNA Interference
  • RNA, Fungal
  • RNA, Small Interfering
  • RNA-Binding Proteins
  • Ribonuclease III
  • Schizosaccharomyces
  • Schizosaccharomyces pombe Proteins