• Register
X
Forgot Password

If you have forgotten your password you can enter your email here and get a temporary password sent to your email.

X

Leaving Community

Are you sure you want to leave this community? Leaving the community will revoke any permissions you have been granted in this community.

No
Yes

GGA1 interacts with the adaptor protein AP-1 through a WNSF sequence in its hinge region.

The Golgi-associated gamma-adaptin-related ADP-ribosylation factor-binding proteins (GGAs) are critical components of the transport machinery that mediates the trafficking of the mannose 6-phosphate receptors and associated cargo from the trans-Golgi network to the endosomes. The GGAs colocalize in vivo with the clathrin adaptor protein AP-1 and bind to AP-1 in vitro, suggesting that the two proteins may cooperate in packaging the mannose 6-phosphate receptors into clathrin-coated vesicles at the trans-Golgi network. Here, we demonstrate that the sequence, (382)WNSF(385), in the hinge region of GGA1 mediates its interaction with the AP-1 gamma-ear. The Trp and Phe constitute critical amino acids in this interaction. The binding of Rabaptin5 to the AP-1 gamma-ear, which occurs through a FXXPhi motif, is inhibited by a peptide encoding the GGA1 (382)WNSF(385) sequence. Moreover, mutations in the AP-1 gamma-ear that abolish its interaction with Rabaptin5 also preclude its association with GGA1. These results suggest that the GGA1 WXXF-type and Rabaptin5 FXXPhi-type motifs bind to the same or highly overlapping sites in the AP-1 gamma-ear. This binding is modulated by residues adjacent to the core motifs.

Pubmed ID: 14973137

Authors

  • Bai H
  • Doray B
  • Kornfeld S

Journal

The Journal of biological chemistry

Publication Data

April 23, 2004

Associated Grants

  • Agency: NCI NIH HHS, Id: R01 CA-08759

Mesh Terms

  • ADP-Ribosylation Factors
  • Adaptor Proteins, Vesicular Transport
  • Amino Acid Motifs
  • Amino Acid Sequence
  • Animals
  • Binding Sites
  • Binding, Competitive
  • Brain
  • COS Cells
  • Carrier Proteins
  • DNA, Complementary
  • Dose-Response Relationship, Drug
  • Escherichia coli
  • Gene Library
  • Glutathione Transferase
  • Humans
  • Immunoblotting
  • In Vitro Techniques
  • Membrane Proteins
  • Molecular Sequence Data
  • Mutation
  • Nerve Tissue Proteins
  • Peptides
  • Phenylalanine
  • Plasmids
  • Protein Binding
  • Protein Structure, Tertiary
  • Receptor, IGF Type 2
  • Sequence Homology, Amino Acid
  • Transcription Factor AP-1
  • Tryptophan
  • Vesicular Transport Proteins
  • trans-Golgi Network