• Register
X
Forgot Password

If you have forgotten your password you can enter your email here and get a temporary password sent to your email.

X

Leaving Community

Are you sure you want to leave this community? Leaving the community will revoke any permissions you have been granted in this community.

No
Yes

Microarray analysis shows that some microRNAs downregulate large numbers of target mRNAs.

MicroRNAs (miRNAs) are a class of noncoding RNAs that post-transcriptionally regulate gene expression in plants and animals. To investigate the influence of miRNAs on transcript levels, we transfected miRNAs into human cells and used microarrays to examine changes in the messenger RNA profile. Here we show that delivering miR-124 causes the expression profile to shift towards that of brain, the organ in which miR-124 is preferentially expressed, whereas delivering miR-1 shifts the profile towards that of muscle, where miR-1 is preferentially expressed. In each case, about 100 messages were downregulated after 12 h. The 3' untranslated regions of these messages had a significant propensity to pair to the 5' region of the miRNA, as expected if many of these messages are the direct targets of the miRNAs. Our results suggest that metazoan miRNAs can reduce the levels of many of their target transcripts, not just the amount of protein deriving from these transcripts. Moreover, miR-1 and miR-124, and presumably other tissue-specific miRNAs, seem to downregulate a far greater number of targets than previously appreciated, thereby helping to define tissue-specific gene expression in humans.

Pubmed ID: 15685193

Authors

  • Lim LP
  • Lau NC
  • Garrett-Engele P
  • Grimson A
  • Schelter JM
  • Castle J
  • Bartel DP
  • Linsley PS
  • Johnson JM

Journal

Nature

Publication Data

February 17, 2005

Associated Grants

None

Mesh Terms

  • 3' Untranslated Regions
  • Base Sequence
  • Brain
  • Down-Regulation
  • Genes, Reporter
  • Humans
  • MicroRNAs
  • Molecular Sequence Data
  • Muscles
  • Mutation
  • Oligonucleotide Array Sequence Analysis
  • Organ Specificity
  • RNA, Messenger
  • Substrate Specificity