Targeted oncogene activation by site-specific recombination in transgenic mice.
Journal:
Proc. Natl. Acad. Sci. U.S.A. 1992 JulAuthors:
Lakso M, Sauer B, Mosinger B, Lee EJ, Manning RW, Yu SH, Mulder KL, Westphal H
Abstract
An efficient and accurate method for controlled in vivo transgene modulation by site-directed recombination is described. Seven transgenic mouse founder lines were produced carrying the murine lens-specific alpha A-crystallin promoter and the simian virus 40 large tumor-antigen gene sequence, separated by a 1.3-kilobase-pair Stop sequence that contains elements preventing expression of the large tumor-antigen gene and Cre recombinase recognition sites. Progeny from two of these lines were mated
...[more]with transgenic mice expressing the Cre recombinase under control of either the murine alpha A-crystallin promoter or the human cytomegalovirus promoter. All double-transgenic offspring developed lens tumors. Subsequent analysis confirmed that tumor formation resulted from large tumor-antigen activation via site-specific, Cre-mediated deletion of Stop sequences.[less]
Mesh Headings:
Animals, Antigens, Polyomavirus Transforming, Base Sequence, Cataract, DNA Nucleotidyltransferases, Eye Neoplasms, Integrases, Mice, Mice, Transgenic, Molecular Sequence Data, Oligodeoxyribonucleotides, Oncogenes, Recombination, Genetic, Regulatory Sequences, Nucleic Acid, Viral Proteins