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Reconstitution of DNA segregation driven by assembly of a prokaryotic actin homolog.

Authors:
Garner EC, Campbell CS, Weibel DB, Mullins RD
Affiliation:
Journal:
Science (New York, N.Y.)

Abstract

Multiple unrelated polymer systems have evolved to partition DNA molecules between daughter cells at division. To better understand polymer-driven DNA segregation, we reconstituted the three-component segregation system of the R1 plasmid from purified components. We found that the ParR/parC complex can construct a simple bipolar spindle by binding the ends of ParM filaments, inhibiting dynamic instability, and acting as a ratchet permitting incorporation of new monomers and riding on the elongating filament ends. Under steady-state conditions, the dynamic instability of unattached ParM filaments provides the energy required to drive DNA segregation.

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