Genetic control of neuronal activity in mice conditionally expressing TRPV1.
Journal:
Nat. Methods 2008 MayAuthors:
Arenkiel BR, Klein ME, Davison IG, Katz LC, Ehlers MD
Abstract
Here we describe a knock-in mouse model for Cre-loxP-based conditional expression of TRPV1 in central nervous system neurons. Expression of Cre recombinase using biolistics, lentivirus or genetic intercrosses triggered heterologous expression of TRPV1 in a cell-specific manner. Application of the TRPV1 ligand capsaicin induced strong inward currents, triggered action potentials and activated stereotyped behaviors, allowing cell type-specific chemical genetic control of neuronal activity in vitro
...[more] and in vivo.[less]
Mesh Headings:
Action Potentials, Animals, Behavior, Animal, Brain, Capsaicin, Gene Targeting, Genes, Reporter, Genetic Vectors, Green Fluorescent Proteins, Immunohistochemistry, Integrases, Lentivirus, Mice, Mice, Knockout, Neurons, Patch-Clamp Techniques, Rats, Recombination, Genetic, TRPV Cation Channels, Transfection