• Register
X
Forgot Password

If you have forgotten your password you can enter your email here and get a temporary password sent to your email.

X

Leaving Community

Are you sure you want to leave this community? Leaving the community will revoke any permissions you have been granted in this community.

No
Yes

Antagonistic regulation of Fus2p nuclear localization by pheromone signaling and the cell cycle.

When yeast cells sense mating pheromone, they undergo a characteristic response involving changes in transcription, cell cycle arrest in early G1, and polarization along the pheromone gradient. Cells in G2/M respond to pheromone at the transcriptional level but do not polarize or mate until G1. Fus2p, a key regulator of cell fusion, localizes to the tip of the mating projection during pheromone-induced G1 arrest. Although Fus2p was expressed in G2/M cells after pheromone induction, it accumulated in the nucleus until after cell division. As cells arrested in G1, Fus2p was exported from the nucleus and localized to the nascent tip. Phosphorylation of Fus2p by Fus3p was required for Fus2p export; cyclin/Cdc28p-dependent inhibition of Fus3p during late G1 through S phase was sufficient to block exit. However, during G2/M, when Fus3p was activated by pheromone signaling, Cdc28p activity again blocked Fus2p export. Our results indicate a novel mechanism by which pheromone-induced proteins are regulated during the transition from mitosis to conjugation.

Pubmed ID: 19188495

Authors

  • Ydenberg CA
  • Rose MD

Journal

The Journal of cell biology

Publication Data

February 9, 2009

Associated Grants

  • Agency: NIGMS NIH HHS, Id: GM37739
  • Agency: NIGMS NIH HHS, Id: R01 GM037739
  • Agency: NIGMS NIH HHS, Id: R01 GM037739-22

Mesh Terms

  • Active Transport, Cell Nucleus
  • CDC28 Protein Kinase, S cerevisiae
  • Cell Cycle
  • Cell Cycle Proteins
  • Cell Nucleus
  • Cyclin-Dependent Kinase Inhibitor Proteins
  • Cytoskeletal Proteins
  • Membrane Proteins
  • Mitogen-Activated Protein Kinases
  • Pheromones
  • Recombinant Fusion Proteins
  • Repressor Proteins
  • Saccharomyces cerevisiae
  • Saccharomyces cerevisiae Proteins
  • Signal Transduction