Floxin, a resource for genetically engineering mouse ESCs.
Journal:
Nat. Methods 2010 JanAuthors:
Singla V, Hunkapiller J, Santos N, Seol AD, Norman AR, Wakenight P, Skarnes WC, Reiter JF
Abstract
We describe a method for the highly efficient and precise targeted modification of gene trap loci in mouse embryonic stem cells (ESCs). Through the Floxin method, gene trap mutations were reverted and new DNA sequences inserted using Cre recombinase and a shuttle vector, pFloxin. Floxin technology is applicable to the existing collection of 24,149 compatible gene trap cell lines, which should enable high-throughput modification of many genes in mouse ESCs.
Mesh Headings:
Alleles, Animals, Attachment Sites, Microbiological, Base Sequence, Cell Line, Embryonic Stem Cells, Enhancer Elements, Genetic, Expressed Sequence Tags, Genetic Engineering, Genetic Markers, Genetic Vectors, Integrases, Mice, Mice, Transgenic, Mutation