A reprogrammable mouse strain from gene-targeted embryonic stem cells.

Journal:

Nat. Methods 2010 Jan

Authors:

Stadtfeld M, Maherali N, Borkent M, Hochedlinger K

Abstract

The derivation of induced pluripotent stem cells (iPSCs) usually involves the viral introduction of reprogramming factors into somatic cells. Here we used gene targeting to generate a mouse strain with a single copy of an inducible, polycistronic reprogramming cassette, allowing for the induction of pluripotency in various somatic cell types. As these 'reprogrammable mice' can be easily bred, they are a useful tool to study the mechanisms underlying cellular reprogramming.

PMID: 20010832

Mesh Headings:

Animals, Blood Cells, Cell Dedifferentiation, Cell Lineage, Cells, Cultured, Collagen, DNA Nucleotidyltransferases, Doxycycline, Embryonic Stem Cells, Fibroblasts, Gene Targeting, Induced Pluripotent Stem Cells, Mice, Mice, Mutant Strains, Mutagenesis, Insertional, Nuclear Reprogramming