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Platelet-derived growth factor receptor beta is critical for zebrafish intersegmental vessel formation.

BACKGROUND: Platelet-derived growth factor receptor beta (PDGFRbeta) is a tyrosine kinase receptor known to affect vascular development. The zebrafish is an excellent model to study specific regulators of vascular development, yet the role of PDGF signaling has not been determined in early zebrafish embryos. Furthermore, vascular mural cells, in which PDGFRbeta functions cell autonomously in other systems, have not been identified in zebrafish embryos younger than 72 hours post fertilization. METHODOLOGY/PRINCIPAL FINDINGS: In order to investigate the role of PDGFRbeta in zebrafish vascular development, we cloned the highly conserved zebrafish homolog of PDGFRbeta. We found that pdgfrbeta is expressed in the hypochord, a developmental structure that is immediately dorsal to the dorsal aorta and potentially regulates blood vessel development in the zebrafish. Using a PDGFR tyrosine kinase inhibitor, a morpholino oligonucleotide specific to PDGFRbeta, and a dominant negative PDGFRbeta transgenic line, we found that PDGFRbeta is necessary for angiogenesis of the intersegmental vessels. SIGNIFICANCE/CONCLUSION: Our data provide the first evidence that PDGFRbeta signaling is required for zebrafish angiogenesis. We propose a novel mechanism for zebrafish PDGFRbeta signaling that regulates vascular angiogenesis in the absence of mural cells.

Pubmed ID: 20593033

Authors

  • Wiens KM
  • Lee HL
  • Shimada H
  • Metcalf AE
  • Chao MY
  • Lien CL

Journal

PloS one

Publication Data

July 1, 2010

Associated Grants

  • Agency: NHLBI NIH HHS, Id: HL096121
  • Agency: NHLBI NIH HHS, Id: R01 HL096121

Mesh Terms

  • Animals
  • Blood Vessels
  • Neovascularization, Physiologic
  • Receptor, Platelet-Derived Growth Factor beta
  • Zebrafish