PMS2 endonuclease activity has distinct biological functions and is essential for genome maintenance.
Journal:
Proc. Natl. Acad. Sci. U.S.A. 2010 JulAuthors:
van Oers JM, Roa S, Werling U, Liu Y, Genschel J, Hou H, Sellers RS, Modrich P, Scharff MD, Edelmann W
Abstract
The DNA mismatch repair protein PMS2 was recently found to encode a novel endonuclease activity. To determine the biological functions of this activity in mammals, we generated endonuclease-deficient Pms2E702K knock-in mice. Pms2EK/EK mice displayed increased genomic mutation rates and a strong cancer predisposition. In addition, class switch recombination, but not somatic hypermutation, was impaired in Pms2EK/EK B cells, indicating a specific role in Ig diversity. In contrast to Pms2-/- mice, P
...[more]ms2EK/EK male mice were fertile, indicating that this activity is dispensable in spermatogenesis. Therefore, the PMS2 endonuclease activity has distinct biological functions and is essential for genome maintenance and tumor suppression.[less]
Mesh Headings:
Adenosine Triphosphatases, Animals, Cells, Cultured, DNA Mismatch Repair, DNA Repair Enzymes, DNA-Binding Proteins, Embryo, Mammalian, Endonucleases, Female, Fertility, Fibroblasts, Genetic Predisposition to Disease, Genomic Instability, Genotype, Humans, Immunoglobulin Class Switching, Immunoglobulin G, Lymphoma, Male, Mice, Mice, Knockout, Mutation, Reverse Transcriptase Polymerase Chain Reaction