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Requirement of JIP1-mediated c-Jun N-terminal kinase activation for obesity-induced insulin resistance.

The c-Jun NH(2)-terminal kinase (JNK) interacting protein 1 (JIP1) has been proposed to act as a scaffold protein that mediates JNK activation. However, recent studies have implicated JIP1 in multiple biochemical processes. Physiological roles of JIP1 that are related to the JNK scaffold function of JIP1 are therefore unclear. To test the role of JIP1 in JNK activation, we created mice with a germ line point mutation in the Jip1 gene (Thr(103) replaced with Ala) that selectively blocks JIP1-mediated JNK activation. These mutant mice exhibit a severe defect in JNK activation caused by feeding of a high-fat diet. The loss of JIP1-mediated JNK activation protected the mutant mice against obesity-induced insulin resistance. We conclude that JIP1-mediated JNK activation plays a critical role in metabolic stress regulation of the JNK signaling pathway.

Pubmed ID: 20679483

Authors

  • Morel C
  • Standen CL
  • Jung DY
  • Gray S
  • Ong H
  • Flavell RA
  • Kim JK
  • Davis RJ

Journal

Molecular and cellular biology

Publication Data

October 10, 2010

Associated Grants

  • Agency: NCI NIH HHS, Id: CA65861
  • Agency: NIDDK NIH HHS, Id: DK032520
  • Agency: NIDDK NIH HHS, Id: DK080742
  • Agency: NIDDK NIH HHS, Id: DK80756
  • Agency: NIDDK NIH HHS, Id: R01 DK080742
  • Agency: NIDDK NIH HHS, Id: R01 DK080756

Mesh Terms

  • Adaptor Proteins, Signal Transducing
  • Adipokines
  • Animals
  • Blood Glucose
  • Cytokines
  • Dietary Fats
  • Energy Metabolism
  • Enzyme Activation
  • Fatty Liver
  • Female
  • Immunoblotting
  • Insulin
  • Insulin Resistance
  • JNK Mitogen-Activated Protein Kinases
  • Male
  • Mice
  • Mice, Inbred C57BL
  • Mice, Knockout
  • Obesity
  • Reverse Transcriptase Polymerase Chain Reaction
  • Signal Transduction