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Dual RMCE for efficient re-engineering of mouse mutant alleles.

Authors:
Osterwalder M, Galli A, Rosen B, Skarnes WC, Zeller R, Lopez-Rios J
Affiliation:
Journal:
Nature methods

Abstract

We have developed dual recombinase-mediated cassette exchange (dRMCE) to efficiently re-engineer the thousands of available conditional alleles in mouse embryonic stem cells. dRMCE takes advantage of the wild-type loxP and FRT sites present in these conditional alleles and in many gene-trap lines. dRMCE is a scalable, flexible tool to introduce tags, reporters and mutant coding regions into an endogenous locus of interest in an easy and highly efficient manner.

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