• Register
X
Forgot Password

If you have forgotten your password you can enter your email here and get a temporary password sent to your email.

X

Leaving Community

Are you sure you want to leave this community? Leaving the community will revoke any permissions you have been granted in this community.

No
Yes

CEP41 is mutated in Joubert syndrome and is required for tubulin glutamylation at the cilium.

Tubulin glutamylation is a post-translational modification that occurs predominantly in the ciliary axoneme and has been suggested to be important for ciliary function. However, its relationship to disorders of the primary cilium, termed ciliopathies, has not been explored. Here we mapped a new locus for Joubert syndrome (JBTS), which we have designated as JBTS15, and identified causative mutations in CEP41, which encodes a 41-kDa centrosomal protein. We show that CEP41 is localized to the basal body and primary cilia, and regulates ciliary entry of TTLL6, an evolutionarily conserved polyglutamylase enzyme. Depletion of CEP41 causes ciliopathy-related phenotypes in zebrafish and mice and results in glutamylation defects in the ciliary axoneme. Our data identify CEP41 mutations as a cause of JBTS and implicate tubulin post-translational modification in the pathogenesis of human ciliary dysfunction.

Pubmed ID: 22246503

Authors

  • Lee JE
  • Silhavy JL
  • Zaki MS
  • Schroth J
  • Bielas SL
  • Marsh SE
  • Olvera J
  • Brancati F
  • Iannicelli M
  • Ikegami K
  • Schlossman AM
  • Merriman B
  • AttiĆ©-Bitach T
  • Logan CV
  • Glass IA
  • Cluckey A
  • Louie CM
  • Lee JH
  • Raynes HR
  • Rapin I
  • Castroviejo IP
  • Setou M
  • Barbot C
  • Boltshauser E
  • Nelson SF
  • Hildebrandt F
  • Johnson CA
  • Doherty DA
  • Valente EM
  • Gleeson JG

Journal

Nature genetics

Publication Data

February 27, 2012

Associated Grants

  • Agency: Medical Research Council, Id: G0700073
  • Agency: Telethon, Id: GGP08145
  • Agency: NICHD NIH HHS, Id: P01 HD070494
  • Agency: NICHD NIH HHS, Id: P01 HD070494-02
  • Agency: NINDS NIH HHS, Id: P30 NS047101
  • Agency: NINDS NIH HHS, Id: P30 NS047101-10
  • Agency: NINDS NIH HHS, Id: P30NS047101
  • Agency: NIDDK NIH HHS, Id: R01 DK068306
  • Agency: NINDS NIH HHS, Id: R01 NS048453
  • Agency: NINDS NIH HHS, Id: R01 NS048453-08
  • Agency: NINDS NIH HHS, Id: R01 NS052455
  • Agency: NINDS NIH HHS, Id: R01 NS052455-06
  • Agency: NINDS NIH HHS, Id: R01 NS064077
  • Agency: NIDDK NIH HHS, Id: R01DK068306
  • Agency: NINDS NIH HHS, Id: R01NS048453
  • Agency: NINDS NIH HHS, Id: R01NS052455
  • Agency: NINDS NIH HHS, Id: R01NS064077
  • Agency: NCRR NIH HHS, Id: S10 RR029130
  • Agency: NCRR NIH HHS, Id: S10 RR029130-01
  • Agency: NIGMS NIH HHS, Id: T32 GM008666
  • Agency: Howard Hughes Medical Institute, Id:

Mesh Terms

  • Animals
  • Centrosome
  • Cerebellar Diseases
  • Chromosome Mapping
  • Cilia
  • Ciliary Motility Disorders
  • Eye Abnormalities
  • Female
  • Genetic Loci
  • Glutamic Acid
  • Humans
  • Male
  • Mice
  • Mutation
  • Peptide Synthases
  • Polycystic Kidney Diseases
  • Polymorphism, Single Nucleotide
  • Protein Processing, Post-Translational
  • Proteins
  • Syndrome
  • Tubulin