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Control of the mitotic exit network during meiosis.

The mitotic exit network (MEN) is an essential GTPase signaling pathway that triggers exit from mitosis in budding yeast. We show here that during meiosis, the MEN is dispensable for exit from meiosis I but contributes to the timely exit from meiosis II. Consistent with a role for the MEN during meiosis II, we find that the signaling pathway is active only during meiosis II. Our analysis further shows that MEN signaling is modulated during meiosis in several key ways. Whereas binding of MEN components to spindle pole bodies (SPBs) is necessary for MEN signaling during mitosis, during meiosis MEN signaling occurs off SPBs and does not require the SPB recruitment factor Nud1. Furthermore, unlike during mitosis, MEN signaling is controlled through the regulated interaction between the MEN kinase Dbf20 and its activating subunit Mob1. Our data lead to the conclusion that a pathway essential for vegetative growth is largely dispensable for the specialized meiotic divisions and provide insights into how cell cycle regulatory pathways are modulated to accommodate different modes of cell division.

Pubmed ID: 22718910

Authors

  • Attner MA
  • Amon A

Journal

Molecular biology of the cell

Publication Data

August 14, 2012

Associated Grants

  • Agency: NIGMS NIH HHS, Id: GM62207
  • Agency: NIGMS NIH HHS, Id: R01 GM056800
  • Agency: NIGMS NIH HHS, Id: R01 GM062207
  • Agency: NIGMS NIH HHS, Id: R29 GM056800
  • Agency: Howard Hughes Medical Institute, Id:

Mesh Terms

  • Cell Cycle Proteins
  • Cytoskeletal Proteins
  • Deoxyribonucleases
  • G1 Phase Cell Cycle Checkpoints
  • GTP-Binding Proteins
  • Gene Expression Regulation, Fungal
  • Meiosis
  • Mitosis
  • Phosphoproteins
  • Protein Binding
  • Protein-Serine-Threonine Kinases
  • Saccharomyces cerevisiae
  • Saccharomyces cerevisiae Proteins
  • Signal Transduction
  • Spores, Fungal
  • tRNA Methyltransferases