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R-loops cause replication impairment and genome instability during meiosis.

R-loops are harmful structures with a negative impact on transcription and recombination during mitosis, but no information exists for meiosis. We used Saccharomyces cerevisiae and Caenorhabditis elegans THO mutants as a tool to determine the consequences of R-loops in meiosis. We found that both S. cerevisiae and C. elegans THO mutants show defective meiosis and an impairment of premeiotic replication as well as DNA-damage accumulation. Importantly, RNase H partially suppressed the replication impairment and the DNA-damage accumulation. We conclude that R-loops can form during meiosis causing replication impairment with deleterious results.

Pubmed ID: 22878416

Authors

  • Castellano-Pozo M
  • GarcĂ­a-Muse T
  • Aguilera A

Journal

EMBO reports

Publication Data

October 1, 2012

Associated Grants

None

Mesh Terms

  • Animals
  • Caenorhabditis elegans
  • Caenorhabditis elegans Proteins
  • DNA Damage
  • DNA Replication
  • DNA, Fungal
  • Genomic Instability
  • Meiosis
  • Nuclear Proteins
  • Ribonuclease H
  • Saccharomyces cerevisiae
  • Saccharomyces cerevisiae Proteins
  • Transcription Factors