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SWI/SNF-like chromatin remodeling factor Fun30 supports point centromere function in S. cerevisiae.

Budding yeast centromeres are sequence-defined point centromeres and are, unlike in many other organisms, not embedded in heterochromatin. Here we show that Fun30, a poorly understood SWI/SNF-like chromatin remodeling factor conserved in humans, promotes point centromere function through the formation of correct chromatin architecture at centromeres. Our determination of the genome-wide binding and nucleosome positioning properties of Fun30 shows that this enzyme is consistently enriched over centromeres and that a majority of CENs show Fun30-dependent changes in flanking nucleosome position and/or CEN core micrococcal nuclease accessibility. Fun30 deletion leads to defects in histone variant Htz1 occupancy genome-wide, including at and around most centromeres. FUN30 genetically interacts with CSE4, coding for the centromere-specific variant of histone H3, and counteracts the detrimental effect of transcription through centromeres on chromosome segregation and suppresses transcriptional noise over centromere CEN3. Previous work has shown a requirement for fission yeast and mammalian homologs of Fun30 in heterochromatin assembly. As centromeres in budding yeast are not embedded in heterochromatin, our findings indicate a direct role of Fun30 in centromere chromatin by promoting correct chromatin architecture.

Pubmed ID: 23028372

Authors

  • Durand-Dubief M
  • Will WR
  • Petrini E
  • Theodorou D
  • Harris RR
  • Crawford MR
  • Paszkiewicz K
  • Krueger F
  • Correra RM
  • Vetter AT
  • Miller JR
  • Kent NA
  • Varga-Weisz P

Journal

PLoS genetics

Publication Data

September 2, 2012

Associated Grants

  • Agency: Biotechnology and Biological Sciences Research Council, Id: BB/F020236/1
  • Agency: Biotechnology and Biological Sciences Research Council, Id:

Mesh Terms

  • Centromere
  • Chromatin Assembly and Disassembly
  • Chromosomal Proteins, Non-Histone
  • Chromosome Segregation
  • DNA-Binding Proteins
  • Heterochromatin
  • Histones
  • Humans
  • Kinetochores
  • Nucleosomes
  • Saccharomyces cerevisiae
  • Saccharomyces cerevisiae Proteins
  • Transcription Factors