Altered cytokine export and apoptosis in mice deficient in interleukin-1 beta converting enzyme.
Journal:
Science 1995 MarAuthors:
Kuida K, Lippke JA, Ku G, Harding MW, Livingston DJ, Su MS, Flavell RA
Abstract
The interleukin-1 beta (IL-1 beta) converting enzyme (ICE) processes the inactive IL-1 beta precursor to the proinflammatory cytokine. Adherent monocytes from mice harboring a disrupted ICE gene (ICE-/-) did not export IL-1 beta or interleukin-1 alpha (IL-1 alpha) after stimulation with lipopolysaccharide. Export of tumor necrosis factor-alpha and interleukin-6 (IL-6) from these cells was also diminished. Thymocytes from ICE-/- mice were sensitive to apoptosis induced by dexamethasone or ionizin
...[more]g radiation, but were resistant to apoptosis induced by Fas antibody. Despite this defect in apoptosis, ICE-/- mice proceed normally through development.[less]
Mesh Headings:
Amino Acid Sequence, Animals, Antibodies, Monoclonal, Antigens, CD95, Antigens, Surface, Apoptosis, Base Sequence, Caspase 1, Cells, Cultured, Chimera, Cysteine Endopeptidases, Cytokines, Dexamethasone, Female, Interleukin-1, Lipopolysaccharides, Male, Mice, Mice, Inbred C57BL, Molecular Sequence Data, Monocytes, Nigericin, T-Lymphocytes