Dominant interfering alleles define a role for c-Myb in T-cell development.
Journal:
Genes Dev. 1994 AprAuthors:
Badiani P, Corbella P, Kioussis D, Marvel J, Weston K
Abstract
The transcription activator c-Myb is expressed at high levels in immature thymocytes and during T-cell activation and may be a regulator of T-cell differentiation. To investigate the role of c-Myb in T-cell development, we generated transgenic mice in which two dominant interfering Myb alleles, one a competitive inhibitor of DNA binding, and the other, an active repressor comprising the Myb DNA-binding domain linked to the Drosophila Engrailed transcription repressor domain, were expressed from
...[more]early times onward in T cells. Both alleles partially blocked thymopoiesis and inhibited proliferation of mature T cells. The Myb-En chimera was the more efficient repressor and might serve as an archetype for the manufacture of other dominant interfering transcription factor alleles.[less]
Mesh Headings:
Alleles, Animals, Base Sequence, Cell Differentiation, Cell Division, DNA-Binding Proteins, Drosophila, Gene Expression Regulation, Developmental, Genes, Dominant, Homeodomain Proteins, Insect Hormones, Lymphocyte Activation, Mice, Mice, Transgenic, Molecular Sequence Data, Proto-Oncogene Proteins, Proto-Oncogene Proteins c-myb, Recombinant Fusion Proteins, Repressor Proteins, Sequence Deletion, Spleen, T-Lymphocytes, Thymus Gland, Transcription Factors, Transcriptional Activation