Complex formation between RAS and RAF and other protein kinases.
Journal:
Proc. Natl. Acad. Sci. U.S.A. 1993 JulAuthors:
Van Aelst L, Barr M, Marcus S, Polverino A, Wigler M
Abstract
We used a Saccharomyces cerevisiae genetic system to detect the physical interaction of RAS and RAF oncoproteins. We also observed interaction between RAS and byr2, a protein kinase implicated as a mediator of the Schizosaccharomyces pombe ras1 protein. Interaction with RAS required only the N-terminal domains of RAF or byr2 and was disrupted by mutations in either the guanine nucleotide-binding or effector-loop domains of RAS. We observed interaction between MEK (a kinase that phosphorylates mi
...[more]togen-activated protein kinases) and the catalytic domain of RAF. RAS and MEK also interacted but only when RAF was overexpressed.[less]
Mesh Headings:
Base Sequence, Fungal Proteins, GTP-Binding Proteins, Guanosine Triphosphate, MAP Kinase Kinase 1, Mitogen-Activated Protein Kinase Kinases, Molecular Sequence Data, Protein Kinases, Protein-Serine-Threonine Kinases, Protein-Tyrosine Kinases, Proto-Oncogene Proteins, Proto-Oncogene Proteins c-raf, Proto-Oncogene Proteins p21(ras), Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins, Schizosaccharomyces, Schizosaccharomyces pombe Proteins, ras Proteins