• Register
X
Forgot Password

If you have forgotten your password you can enter your email here and get a temporary password sent to your email.

X

Leaving Community

Are you sure you want to leave this community? Leaving the community will revoke any permissions you have been granted in this community.

No
Yes

Apoptosis of nur77/N10-transgenic thymocytes involves the Fas/Fas ligand pathway.

The orphan nuclear receptor Nur77/N10 has recently been demonstrated to be involved in apoptosis of T cell hybridomas. We report here that chronic expression of Nur77/N10 in thymocytes of transgenic mice results in a dramatic reduction of CD4+CD8+ double-positive as well as CD4+CD8- and CD4-CD8+ single-positive cell populations due to an early onset of apoptosis. CD4-CD8- double-negative and CD25+ precursor cells, however, are unaffected. Moreover, nur77/N10-transgenic thymocytes show increased expression of Fas ligand (FasL), while the levels of the Fas receptor (Fas) are not increased. The mouse spontaneous mutant gld (generalized lymphoproliferative disease) carries a point mutation in the extracellular domain of the FasL gene that abolishes the ability of FasL to bind to Fas. Thymuses from nur77/N10-transgenic mice on a gld/gld background have increased cellularity and an almost normal profile of thymocyte subpopulations. Our results demonstrate that one pathway of apoptosis triggered by Nur77/N10 in double-positive thymocytes occurs through the upregulation of FasL expression resulting in increased signaling through Fas.

Pubmed ID: 8643610

Authors

  • Weih F
  • Ryseck RP
  • Chen L
  • Bravo R

Journal

Proceedings of the National Academy of Sciences of the United States of America

Publication Data

May 28, 1996

Associated Grants

None

Mesh Terms

  • Animals
  • Antigens, CD2
  • Antigens, CD95
  • Apoptosis
  • Atrophy
  • Base Sequence
  • CD4-Positive T-Lymphocytes
  • CD8-Positive T-Lymphocytes
  • DNA Primers
  • DNA-Binding Proteins
  • Fas Ligand Protein
  • Flow Cytometry
  • Growth Hormone
  • Humans
  • Ligands
  • Membrane Glycoproteins
  • Mice
  • Mice, Transgenic
  • Molecular Sequence Data
  • Nuclear Receptor Subfamily 4, Group A, Member 1
  • Polymerase Chain Reaction
  • RNA, Messenger
  • Receptors, Cytoplasmic and Nuclear
  • Receptors, Steroid
  • T-Lymphocytes
  • Thymus Gland
  • Transcription Factors
  • Transcription, Genetic