Murine caspase-11, an ICE-interacting protease, is essential for the activation of ICE.
Journal:
Cell 1998 FebAuthors:
Wang S, Miura M, Jung YK, Zhu H, Li E, Yuan J
Abstract
We report here the inactivation of a member of the Ice/Ced-3 (caspase) family of cell death genes, casp-11, by gene targeting. Like Ice-deficient mice, casp-11 mutant mice are resistant to endotoxic shock induced by lipopolysaccharide. Production of both IL-1alpha and IL-1beta after lipopolysaccharide stimulation, a crucial event during septic shock and an indication of ICE activation, is blocked in casp-11 mutant mice. casp-11 mutant embryonic fibroblast cells are resistant to apoptosis induced
...[more] by overexpression of ICE. Furthermore, we found that pro-caspase-11 physically interacts with pro-ICE in cells, and the expression of casp-11 is essential for activation of ICE. Our data suggest that caspase-11 is a component of ICE complex and is required for the activation of ICE.[less]
Mesh Headings:
Animals, Apoptosis, Caspase 1, Caspases, Cells, Cultured, Cysteine Endopeptidases, Embryo, Mammalian, Embryonic and Fetal Development, Endopeptidases, Enzyme Activation, Gene Expression Regulation, Developmental, Gene Expression Regulation, Enzymologic, Mice, Mice, Inbred C57BL, Mice, Mutant Strains, Mutagenesis, Shock, Septic